DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
Identifieur interne : 003B79 ( Main/Exploration ); précédent : 003B78; suivant : 003B80DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
Auteurs : Alfredo De La Escosura-Muniz [Espagne] ; Maria Begona Gonzalez-Garcia [Espagne] ; Agustin Costa-Garcia [Espagne]Source :
- Biosensors & bioelectronics [ 0956-5663 ] ; 2007.
Descripteurs français
- Pascal (Inist)
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- topic : Argent.
English descriptors
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Abstract
In this work, a gold complex is used as electroactive label for monitoring hybridization assays on glassy carbon electrodes. Ionic gold is bound to a 30-mer sequence of the SARS (severe acute respiratory syndrome) virus, responsible for the atypical pneumonia, using sodium aurothiomalate. In order to label this single strand, a mixture of sodium aurothiomalate and the strand is prepared. Then, it is incubated for 24 h at 37 °C and, finally, free gold is separated from the labeled strand by a dialysis against a 0.15 M NaCI solution (pH 7.5). The DNA hybridization sensor is designed immobilizing the complementary probe on the pre-treated electrode surface and, then, the hybridization reaction takes place with the gold labeled strand. The electrochemical determination is based on the catalytic effect of electrodeposited gold on the reduction of silver ions. In non-stringent experimental conditions, a limit of detection of 15 fmol (30 μL) is obtained, and discrimination between a complementary oligonucleotide and a three-based mismatch complementary oligonucleotide is achieved. For the discrimination of a single-base mismatch, is needed to use stringent conditions (50% of formamide in the hybridization buffer).
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y Analítica, Universidad de Oviedo</s1><s2>33006 Oviedo, Asturias</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>33006 Oviedo, Asturias</wicri:noRegion></affiliation></author><author><name sortKey="Costa Garcia, Agustin" sort="Costa Garcia, Agustin" uniqKey="Costa Garcia A" first="Agustin" last="Costa-Garcia">Agustin Costa-Garcia</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Deparlamento de Quimica Fisica y Analítica, Universidad de Oviedo</s1><s2>33006 Oviedo, Asturias</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>33006 Oviedo, Asturias</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0281849</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0281849 INIST</idno><idno type="RBID">Pascal:07-0281849</idno><idno 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Analítica, Universidad de Oviedo</s1><s2>33006 Oviedo, Asturias</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>33006 Oviedo, Asturias</wicri:noRegion></affiliation></author><author><name sortKey="Begona Gonzalez Garcia, Maria" sort="Begona Gonzalez Garcia, Maria" uniqKey="Begona Gonzalez Garcia M" first="Maria" last="Begona Gonzalez-Garcia">Maria Begona Gonzalez-Garcia</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Deparlamento de Quimica Fisica y Analítica, Universidad de Oviedo</s1><s2>33006 Oviedo, Asturias</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>33006 Oviedo, Asturias</wicri:noRegion></affiliation></author><author><name sortKey="Costa Garcia, Agustin" sort="Costa Garcia, Agustin" uniqKey="Costa Garcia A" first="Agustin" last="Costa-Garcia">Agustin Costa-Garcia</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Deparlamento de Quimica Fisica y Analítica, Universidad de Oviedo</s1><s2>33006 Oviedo, Asturias</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>33006 Oviedo, Asturias</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Biosensors & bioelectronics</title><title level="j" type="abbreviated">Biosens. bioelectron.</title><idno type="ISSN">0956-5663</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Biosensors & bioelectronics</title><title level="j" type="abbreviated">Biosens. bioelectron.</title><idno type="ISSN">0956-5663</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensor</term><term>Carbon electrode</term><term>DNA</term><term>Molecular hybridization</term><term>Severe acute respiratory syndrome</term><term>Silver</term><term>Sodium aurothiomalate</term><term>Virus</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Hybridation moléculaire</term><term>Electrode carbone</term><term>Argent</term><term>Aurothiomalate de sodium</term><term>Virus</term><term>Syndrome respiratoire aigu sévère</term><term>DNA</term><term>Biodétecteur</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Argent</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this work, a gold complex is used as electroactive label for monitoring hybridization assays on glassy carbon electrodes. Ionic gold is bound to a 30-mer sequence of the SARS (severe acute respiratory syndrome) virus, responsible for the atypical pneumonia, using sodium aurothiomalate. In order to label this single strand, a mixture of sodium aurothiomalate and the strand is prepared. Then, it is incubated for 24 h at 37 °C and, finally, free gold is separated from the labeled strand by a dialysis against a 0.15 M NaCI solution (pH 7.5). The DNA hybridization sensor is designed immobilizing the complementary probe on the pre-treated electrode surface and, then, the hybridization reaction takes place with the gold labeled strand. The electrochemical determination is based on the catalytic effect of electrodeposited gold on the reduction of silver ions. In non-stringent experimental conditions, a limit of detection of 15 fmol (30 μL) is obtained, and discrimination between a complementary oligonucleotide and a three-based mismatch complementary oligonucleotide is achieved. For the discrimination of a single-base mismatch, is needed to use stringent conditions (50% of formamide in the hybridization buffer).</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><country name="Espagne"><noRegion><name sortKey="De La Escosura Muniz, Alfredo" sort="De La Escosura Muniz, Alfredo" uniqKey="De La Escosura Muniz A" first="Alfredo" last="De La Escosura-Muniz">Alfredo De La Escosura-Muniz</name></noRegion><name sortKey="Begona Gonzalez Garcia, Maria" sort="Begona Gonzalez Garcia, Maria" uniqKey="Begona Gonzalez Garcia M" first="Maria" last="Begona Gonzalez-Garcia">Maria Begona Gonzalez-Garcia</name><name sortKey="Costa Garcia, Agustin" sort="Costa Garcia, Agustin" uniqKey="Costa Garcia A" first="Agustin" last="Costa-Garcia">Agustin Costa-Garcia</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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